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It is not meant to be complete. Thus, scientists, doctors, researchers and other users are able to perform TCSPC FLIM microscopy, Multi-Wavelength FLIM, Simultaneous FLIM and Fast-Acquisition FLIM easily. Choose from FLIM systems to upgrade one photon and multiphoton microscopes, for confocal or non-descanned detection. Please see The bh TCSPC Handbook, 8th edition, 2019. TCSPC FLIM in the wavelength range from 800 nm to 1700 nm (Conference Presentation) - NASA/ADS TCSPC FLIM in the wavelength range from 800 nm to 1700 nm (Conference Presentation) The FLIMera is designed for use with HORIBA’s highly intuitive EzTime Image software. This software is used for FLIMera control, data acquisition and analysis.
Video rate FLIM with pixel-wise TCSPC. A New Concept in FLIM Imaging. FLIMera Camera. The HORIBA FLIMera camera is a new concept in.
Modular Systems Unsurpassed in Time Resolution. Becker & Hickl continues to advance techniques of Time-Correlated Single Photon Counting (TCSPC) and Fluorescence Lifetime Imaging Microscopy (FLIM) with its family of high performance, high speed electronic modules.
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Advanced Search >. Home > Proceedings > Volume 10882 > > Proceedings > Volume Imaging techniques based on time-correlated single photon counting (TCSPC), such as fluorescence lifetime imaging microscopy (FLIM), rely on fast single-photon detectors as well as timing electronics in the form of time-to-digital or time-to-analog converters. Conventional systems rely on stand-alone or small arrays (up to 32) of detectors and external timing and memory modules.
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resolves multi-exponential decay functions into their components, is able to record multi-wavelength TCSPC-based lightsheet FLIM images of cancer cell spheroids with two different fluorescent labels, one throughout the spheroid and one on the surface only. (A) xy FLIM image, with the color-encoded fluorescence lifetime contrast given by the color scale bar on the left, (B) xz FLIM image, (C) yz FLIM image, and (D) xy fluorescence intensity image, with a 100 μm scale bar to indicate spatial Get flim_data_stack and intensity_image from raw TTTR data. Note: once NEXT CODE block is executed raw TTTR data variables (sync, tcspc, channel, special) are deleted. flim_data_stack: (pixX, pixY, spectral_detection_channel, tcspc_bins) Phasor FLIM is a very powerful analysis tool for molecular species separation and FRET analysis, in particular when the donor has a multi-exponential lifetime, something which is typical of CFP [cyan fluorescent protein] (Caiolfa et al., 2007). The Leica D FLIM an MP FLIM systems are add-ons of the TCS SP2 and TCS SP5 laser scanning microscopes.
This software is used for FLIMera control, data acquisition and analysis. FLIMera provides 4 modes of operation.
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When installed on a Scientifica multiphoton microscope, the FLIM upgrade enables simultaneous fluorescence intensity and fluorescence lifetime imaging, in up to two colour channels, using the versatile Picoquant TCSPC system. Its advantages, when compared to the TCSPC are twofold: a) the short time required for data acquisition; and b) the higher sensitivity of the technique (due to the 100% duty-cycle). Alternatively, FLIM data can be acquired using the TCSPC method, or both methodologies can be implemented on the same instrument.
Thus, scientists, doctors, researchers and other users are able to perform TCSPC FLIM microscopy, Multi-Wavelength FLIM, Simultaneous FLIM and Fast-Acquisition FLIM easily. Choose from FLIM systems to upgrade one photon and multiphoton microscopes, for confocal or non-descanned detection.
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Fluorescence-lifetime imaging microscopy or FLIM is an imaging technique based on the differences in the exponential decay rate of the fluorophore from a sample. . It can be used as an imaging technique in confocal microscopy, two-photon excitation microscopy, and multiphoton tomograp Fluorescence Lifetime Imaging (FLIM) produces an image based on the differences in the excited state decay rate from a fluorescent sample. Thus, FLIM is a fluorescence imaging technique where the contrast is based on the lifetime of individual fluorophores rather than their emission spectra.